We created a phage display heavy chain antibody library from non-immunized llama blood. It was very easy to determine library size but we are having a great deal of difficulty trying to figure out how to determine diversity. We sequenced 200 isolates from the library to provide data to help in diversity determination but aren’t certain how this can be done reliably with a non-immunized library. The literature is really vague about how diversity is calculated and often just mentions it was calculated but not how it was calculated. I queried the CDC bio-statistician list serve and one member suggested capture-recapture. I don’t know anything about this technique or even if this really is the best methodology. Any advice or suggestions (especially for someone who does not have a strong background in unusual statistical analysis)?
Any help will be apprecited.
I didn’t find the right solution from the Internet.
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